Subsecond release of catecholamines from CD4+ T lymphocytes

Abstract The neuroimmune system is complex and regulated by small chemical messengers released from resident immune neuronal cells. Catecholamines like dopamine norepinephrine are important responsible for initiating propagating messages between neurons cells; however, the mechanism dynamics of this signaling relatively unknown. presence intracellular machinery to synthesize interact with catecholamines in T lymphocytes has been known over a decade; direct measurement catecholamine secretion real time cells not possible. Here, we have discovered that CD4+ capable releasing on subsecond scale. We used revolutionary technique, often neuroscience field, called fast-scan cyclic voltammetry (FSCV) measure fluctuations isolated This work provides evidence catecholamines; potentially as feedback communicator sympathetic host organs. These results also prove can signal same scale . will hopefully open door enable more sophisticated studies at neuron-immune synapse future. National Institute Health R01AI151552